...isolate mycelium from a mushroom!
You want to isolate mycelium from a mushroom and you don't know how? Maybe I can help.
1- First you need to choose a mushroom (in this photo Pleurotus ostreatus).
2 - Then open the mushroom by its cap.
3 - Choose your isolation spot (use a part of the mushroom stem or cap for the isolation)
4 - With a scalpel cut a piece of the mushroom in your isolation spot.
5 - Put that mushroom piece in a Petri dish with the right culture media (the culture media varies according to the mushroom you're using)
6 - Put the Petri dish in a place with the right temperature of incubation so the mycelium can grow.
And there you go! A nice and hopefully contamination-free isolation.