Monday, 15 October 2012

How to...

...isolate mycelium from a mushroom!


You want to isolate mycelium from a mushroom and you don't know how? Maybe I can help.


1- First you need to choose a mushroom (in this photo Pleurotus ostreatus).

 2 - Then open the mushroom by its cap.

3 - Choose your isolation spot (use a part of the mushroom stem or cap for the isolation)

 4 - With a scalpel cut a piece of the mushroom in your isolation spot.

5 - Put that mushroom piece in a Petri dish with the right culture media (the culture media varies according to the mushroom you're using)

6 - Put the Petri dish in a place with the right temperature of incubation so the mycelium can grow.


And there you go! A nice and hopefully contamination-free isolation. 



Note: If you don't have access to a lab you can use an alcohol lamp to keep the isolation environment as sterile as possible.